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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
12/11/2020 |
Data da última atualização: |
23/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GRUNENNVALDT, R. L.; DEGENHARDT, J.; BROOKS, P.; TOMASI, J. de C.; HANSEL, F. A.; TRAN, T.; GOMES, E. N.; DESCHAMPS, C. |
Afiliação: |
RENATA LÚCIA GRUNENNVALDT, UFPR; JULIANA DEGENHARDT, CNPF; PETER BROOKS, University of The Sunshine Coast; JÉSSICA DE CÁSSIA TOMASI, UFPR; FABRICIO AUGUSTO HANSEL, CNPF; TRONG TRAN, University of The Sunshine Coast; ERIK N. GOMES, The State University of New Jersey; CÍCERO DESCHAMPS, UFPR. |
Título: |
Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype infl uence, medium optimization and compounds identifi cation. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências , v. 92, n. 3, e20181251, 2020. 16 p. |
DOI: |
https://doi.org/10.1590/0001-3765202020181251 |
Idioma: |
Inglês |
Conteúdo: |
Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine. |
Palavras-Chave: |
Acúmulo de biomassa; Atividade antioxidante; Biomass accumulation; Erva mate; Plant growth regulators. |
Thesagro: |
Ilex Paraguariensis; Regulador de Crescimento; Sacarose. |
Thesaurus Nal: |
Antioxidant activity; Sucrose; Yerba mate. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217736/1/Juliana-Grunennvaldt-et-al-2020.pdf
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Marc: |
LEADER 02511naa a2200349 a 4500 001 2126527 005 2020-11-23 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/0001-3765202020181251$2DOI 100 1 $aGRUNENNVALDT, R. L. 245 $aCallus culture as a new approach for the production of high added value compounds in Ilex paraguariensis$bgenotype infl uence, medium optimization and compounds identifi cation.$h[electronic resource] 260 $c2020 520 $aIlex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine. 650 $aAntioxidant activity 650 $aSucrose 650 $aYerba mate 650 $aIlex Paraguariensis 650 $aRegulador de Crescimento 650 $aSacarose 653 $aAcúmulo de biomassa 653 $aAtividade antioxidante 653 $aBiomass accumulation 653 $aErva mate 653 $aPlant growth regulators 700 1 $aDEGENHARDT, J. 700 1 $aBROOKS, P. 700 1 $aTOMASI, J. de C. 700 1 $aHANSEL, F. A. 700 1 $aTRAN, T. 700 1 $aGOMES, E. N. 700 1 $aDESCHAMPS, C. 773 $tAnais da Academia Brasileira de Ciências$gv. 92, n. 3, e20181251, 2020. 16 p.
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Embrapa Florestas (CNPF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
22/02/2002 |
Data da última atualização: |
22/02/2002 |
Autoria: |
FELFILI, J. M.; FRANCO, A. C.; FAGG, C. W.; SOUSA-SILVA, J. C. |
Título: |
Desenvolvimento inicial de especies de mata de galeria. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
In: RIBEIRO, J. F.; FONSECA, C. E. L. da; SOUSA-SILVA, J. C. (Ed.). Cerrado: caracterizacao e recuperacao de matas de galeria. Planaltina: Embrapa Cerrados, 2001. |
Páginas: |
p.779-811. |
Idioma: |
Português |
Conteúdo: |
Determinantes, padroes e processos naturais nas matas de galeria; Estrutura e composicao floristica de matas de galeria; Ecofisiologia de plantas nativas; Os efeitos do deficit hidrico e da tolerancia a inundacao; Efeitos de baixas temperaturas; Efeitos de nutrientes; Desenvolvimento inicial sob diferentes condicoes de sombreamento. |
Palavras-Chave: |
Riparian vegetation. |
Thesagro: |
Cerrado; Mata Ciliar. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00984naa a2200205 a 4500 001 1564308 005 2002-02-22 008 2001 bl uuuu u00u1 u #d 100 1 $aFELFILI, J. M. 245 $aDesenvolvimento inicial de especies de mata de galeria. 260 $c2001 300 $ap.779-811. 520 $aDeterminantes, padroes e processos naturais nas matas de galeria; Estrutura e composicao floristica de matas de galeria; Ecofisiologia de plantas nativas; Os efeitos do deficit hidrico e da tolerancia a inundacao; Efeitos de baixas temperaturas; Efeitos de nutrientes; Desenvolvimento inicial sob diferentes condicoes de sombreamento. 650 $aCerrado 650 $aMata Ciliar 653 $aRiparian vegetation 700 1 $aFRANCO, A. C. 700 1 $aFAGG, C. W. 700 1 $aSOUSA-SILVA, J. C. 773 $tIn: RIBEIRO, J. F.; FONSECA, C. E. L. da; SOUSA-SILVA, J. C. (Ed.). Cerrado: caracterizacao e recuperacao de matas de galeria. Planaltina: Embrapa Cerrados, 2001.
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